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- Primary Antibodies ›
- Tyrosine Hydroxylase Antibodies
Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
View all (68) Tyrosine Hydroxylase antibodies
Cite Tyrosine Hydroxylase Monoclonal Antibody (185)
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Tyrosine Hydroxylase Antibody (MA1-24654) in ICC/IF
Immunofluorescence analysis of Tyrosine Hydroxylase using of Tyrosine Hydroxylase using Tyrosine Hydroxylase Monoclonal Antibody (185) (Product # MA1-24654) was performed using 70% confluent log phase PC-12 cells treated with 5 µg/mL dexamethasone for 6 days. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 min... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
MA1-24654
Applications
Tested Dilution
Publications
Western Blot(WB)
1:25-1:50
-
Immunohistochemistry (IHC)
-
View 8 publications8 publications
Immunohistochemistry (Paraffin) (IHC (P))
Assay-dependent
-
Immunohistochemistry (Frozen)(IHC (F))
Assay-dependent
-
Immunocytochemistry(ICC/IF)
1:50
View 2 publications2 publications
Product Specifications
Species Reactivity
Human,Mouse,Rat
Published species
Human,Mouse,Rat
Host/Isotype
Mouse/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
185
Immunogen
Recombinant protein corresponding to the C-terminal portion of mouse Tyrosine Hydroxylase.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.24 mg/mL
Storage buffer
tissue culture supernatant
Contains
15mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_795666
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Tyrosine hydroxylase (TH) is an enzyme involved in the synthesis of catecholamine neurotransmitters dopamine, epinephrine, and norepinephrine. In all species, catecholamine synthesis is regulated by the interaction of TH with a cofactor, tetrahydrobiopterin (BH4). BH4 binds to the TH catalytic domain, resulting in enzymatic activity. Unlike TH in non-primate species, four human TH mRNA splice variants (hTH1-hTH4) have been isolated. These variants are identical in their catalytic domain, but differ in their N-terminal, regulatory domains. TH is also responsible for the conversion of L-tyrosine to L-dopa. TH plays a key role in the physiology of adrenergic neurons. The role of TH in the synthesis of catecholamine neurotransmitters suggests a correlation between the enzyme and a number of neuropathogenic diseases including: Parkinson's disease, schizophrenia, Segawa syndrome, and dystonia, as well as a variety of cardiovascular diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases:dystonia 14; EC 1.14.16.2; HGNC:11782; TH; TH isoform 3; TH isoform a; TH-4; TY3H; TYH antibody; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase
UniProt ID:(Human) P07101, (Mouse) P24529, (Rat) P04177
Entrez Gene ID:(Human) 7054, (Mouse) 21823, (Rat) 25085
Function(s)
response to hypoxiasynaptic transmission, dopaminergicdopamine biosynthetic process from tyrosineheart developmentvisual perceptionlearningmemorymating behaviorlocomotory behaviorregulation of heart contractionaromatic amino acid family metabolic processorgan morphogenesisaminergic neurotransmitter loading into synaptic vesicleneurotransmitter biosynthetic processdopamine biosynthetic processepinephrine biosynthetic processnorepinephrine biosynthetic processcatecholamine biosynthetic processeye photoreceptor cell developmenteating behaviorresponse to ethanolembryonic camera-type eye morphogenesisoxidation-reduction processresponse to amphetamineheart morphogenesisfatty acid metabolic processsphingolipid metabolic processsensory perception of soundresponse to water deprivationresponse to light stimulusresponse to herbicideresponse to salt stressanatomical structure morphogenesisresponse to zinc ionmulticellular organism agingresponse to activityglycoside metabolic processphthalate metabolic processcerebral cortex developmentresponse to nutrient levelsresponse to estradiolresponse to lipopolysaccharideisoquinoline alkaloid metabolic processsocial behaviorcellular response to drugresponse to isolation stressresponse to immobilization stressterpene metabolic processcircadian sleep/wake cycleresponse to peptide hormonepigmentationresponse to etherresponse to pyrethroidresponse to corticosteroneresponse to electrical stimulusphytoalexin metabolic processcellular response to manganese ioncellular response to nicotinecellular response to glucose stimuluscellular response to growth factor stimulusresponse to metal ionresponse to organic cyclic compoundresponse to insecticideresponse to nicotineresponse to drugresponse to steroid hormonecognitionprotein hom*otetramerizationresponse to growth factorcellular response to alkaloid
Process(es)
tyrosine 3-monooxygenase activityprotein bindingferrous iron bindingferric iron bindingamino acid bindingoxygen bindingenzyme bindingprotein domain specific bindingtetrahydrobiopterin bindingdopamine bindingmonooxygenase activityiron ion bindingoxidoreductase activityoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygenmetal ion binding
It has to be done as per old AB suggested Products section.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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